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KMID : 0387720150260010026
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Korean Journal of Blood Transfusion
2015 Volume.26 No. 1 p.26 ~ p.37
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A Feasibility Study for Cryopreserving Peripheral Blood Stem Cell Collects at High Cell Concentration
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박종은:Park Jong-Eun
정혜경:Jung Hae-Kyung/박혜경:Park Hye-Kyung/이승태:Lee Seung-Tae/김석진:Kim Seok-Jin/장준호:Jang Jun-Ho/김기현:Kim Ki-Hyun/김원석:Kim Won-Suk/정철원:Jung Chul-Won/강은숙:Kang Eun-Suk/김대원:kim Dae-Won
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Abstract
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Background: For autologous hematopoietic stem cell transplantation (HSCT), the volume of infused and DMSO contained in graft are the major causes of complications related to infusion. In this study, we evaluated feasibility of cryopreserving peripheral blood stem cell collects (PBSCC) at high cell concentration.
Methods: PBSCC from 40 patients with multiple myeloma or lymphoma were split and cryopreserved at two different concentrations of TNCs; one for standard concentration (SC) (2×108 cells/mL) and the other for high concentration (HC) (3×108 cells/mL). The viability of total nucleated cells and CD34＋ cell count were examined before cryopreservation and after thawing. CFU-GM was examined with thawed products. Data were analyzed as two groups between good mobilizer (GM) and poor mobilizer (PM).
Results: There were no differences in TNC viability between SC and HC of all patients (P=0.0656) and PM (P=0.9658), however HC of GM showed significantly lower viability than SC (P=0.0314). CD34＋ cell viability did not differ between SC and HC. CD34＋ cell recovery was decreased in HC of all patients (P=0.459) and GM (P=0.0164), but no differences between SC and HC in PM (P=0.9658). CFU-GM clonogenic efficiency between SC and HC was not different in all patients (P=0.0635) and PM (P=0.8984), but was decreased in HC of GM (P=0.0427).
Conclusion: Cryopreservation of PBSCC at 3×108 cells/mL seems to have minimal adverse effect on the quality of PBSC after thawing, particularly in PM. This approach may help to reduce infusion related complications while decreasing the cost of processing and storage of PBSCC.
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KEYWORD
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CD34 antigen, Cryopreservation, Peripheral blood stem cell transplantation
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